… human suspension packaging cell line producing ecotropic retroviral MLV (PVC-211) vectors efficiently transducing murine hematopoietic stem and progenitor cells

Y van Heuvel, K Berg, T Hirch, K Winn… - Journal of virological …, 2021 - Elsevier
… Despite their inability to transduce non-cycling cells, retroviral vectors derived from MLV …
(LTRs; dotted arrows) of murine embryonic stem cell virus (MSCV) and the packaging signal Ψ …

[HTML][HTML] Potent and reversible lentiviral vector restriction in murine induced pluripotent stem cells

FK Geis, M Galla, D Hoffmann, J Kuehle, D Zychlinski… - Retrovirology, 2017 - Springer
… suitable alternative to embryonic stem cells (ESC) for the study of cellular differentiation and
… tested retroviral vectors were observed. Notably, both GV and LV exhibited low transduction

Retroviral transduction of quiescent murine hematopoietic stem cells

CS Park, HD Lacorazza - Cellular Quiescence: Methods and Protocols, 2018 - Springer
… toward transducing stem cells was the selection of viral vectors. … murine and human
hematopoietic and embryonic stem cells [7,8,… with a retroviral vector and ecotropic viral envelope. …

[HTML][HTML] Ecotropic HIV-1 vectors pseudotyped with R-peptide-deleted envelope protein variants reveal improved gene transfer efficiencies

N Tschorn, C Söhngen, K Berg, J Stitz - Virology, 2022 - Elsevier
… To elevate gene transduction, retroviral vectors are commonly … HSCs and progenitor cells
mediated by ecotropic MLV vectors … To improve gene transduction efficiencies, retroviral Env …

Pseudotyped lentiviral vectors: one vector, many guises

AV Joglekar, S Sandoval - Human gene therapy methods, 2017 - liebertpub.com
… Viruses can be repurposed into viral vectors to achieve … vectors can transduce human
CD34+ hematopoietic stem/… cells such as murine embryonic stem cells, human HSCs, and …

Construction of a replication-competent retroviral vector for expression of the VSV-G envelope glycoprotein for cancer gene therapy

SY Jin, YT Jung - Archives of Virology, 2020 - Springer
Transduction of human hematopoietic stem cells with GALV … 1, the ‘ecotropic envelope
glycoprotein’-encoding gene from … retroviral vectors in transducing human cancer cell lines. Since …

Optimization of retroviral packaging cells for scale-up of vector production

Y van Heuvel - 2023 - repo.uni-hannover.de
… design of cell line establishment and retroviral vector- … in genomic DNA of transduced
COS7mCAT target cells. ................… ; dotted arrows) of murine embryonic stem cell virus (MSCV) and …

[HTML][HTML] Evaluating the safety of retroviral vectors based on insertional oncogene activation and blocked differentiation in cultured thymocytes

S Zhou, S Fatima, Z Ma, YD Wang, T Lu, LJ Janke… - Molecular Therapy, 2016 - cell.com
transduction of thymic progenitor cells with murine gamma-RV or HIV-derived lentiviral vectors
… insertions as has been noted in certain human SCID gene therapy trials. This system was …

Introducing genes into mammalian cells: viral vectors

M Sena-Esteves, G Gao - Cold Spring Harbor Protocols, 2020 - cshprotocols.cshlp.org
… 2-293 cell line sold by Clontech generates ecotropic … allow for selection of retrovirus
vector-transduced cells in culture. (… stem/progenitor cells with retrovirus or lentivirus vectors is …

Development of transposon and retrovirus-derived vectors for the rapid establishment of efficient producer cell lines

N Tschorn - 2023 - repo.uni-hannover.de
… this, viral vectors were equipped with the ecotropic envelope … lines in relation to retroviral
gene transduction is provided in … optimize transposon vectors and retroviral vectors enabling …